Targeting CD70 using CAR NK cells to enhance NK cells cytolytic effect against osteosarcoma

Abstract Background: Cure rates for osteosarcoma (OS) have not improved in >30 years and no new effectivetherapies been identified metastatic disease. Natural killer (NK) cells become an attractive tool cancer immunotherapy. Efficay is by direct tumor targeting introducing a specific chimeric antigen receptor (CAR). Emerging knowledge suggests CD70 to be viable candidate as expression higher lung metastases it has correlated with escape from immune surveillance. Objective: To assess whether using CAR-NK we can enhance trafficking, homing proliferation of NK therefore therapeutic efficacy against OS. Methods: We analyzed the cBioportal database verify Surface on human OS cell lines normal osteoblasts was determined flow cytometry. CAR constructs were sequenced, verified transduced into cells. control cytolytic activity evaluated IncuCyte. In vitrocytokine release measured upon NK/NK exposure (IsoPlexis). Results: Database analysis confirmed gene amplification. Flow cytometry showed variable osteoblasts. There increase percent lysis at highest effector:target ratios compared found predominant chemoattractive/stimulatory cytokines Conclusion: immunotherapy potential increased vitro. Cytolytic may influenced cytokines.